Composite
Part:BBa_K3096055:Design
Designed by: Patrick Mueller, Jakob Keck, Lina Widerspick, Famke Baeuerle Group: iGEM19_Tuebingen (2019-10-15)
Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Removal of unwanted restriction sites.
Source
Combination of two previously designed parts.