Composite

Part:BBa_K3096055:Design

Designed by: Patrick Mueller, Jakob Keck, Lina Widerspick, Famke Baeuerle   Group: iGEM19_Tuebingen   (2019-10-15)


Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removal of unwanted restriction sites.


Source

Combination of two previously designed parts.

References